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1.
Article in English | IMSEAR | ID: sea-162694

ABSTRACT

Cement industry accounts for the second largest emitter of anthropogenic greenhouse gas in the globe with 900 kg CO2 emitted into the atmosphere from producing one tonne of cement. Hence, the effort made to mitigate this issue seems not productive , which gives rise to the design of the carbon capture and sequestration [CCS] process which is one of the few ways obtained to greatly reduce CO2 production from the cement plant. The research work assessed the technology used for the cement plant by employing an old cement plant with post-combustion CO2 capture using physical solvent (Selexol). The Aspen Hysys simulation results show that the process can capture 97% of the CO2 and lean loading of 0.37. The Ashaka Cement Plant operates at maximum capacity of approx. 1 million tonnes cement /year with CO2 released at about 500,000 tonnes per year. The capture unit was able to reduce the CO2 released into the atmosphere from 4.86% to 0.13%. The overall result of the analysis shows that selexol has proven to be thermally and chemically stable under the operating conditions used. It is recommended that, the simulation results should be retrofitted into the Ashaka cement plant, in order to determine the best CO2 capture efficiency, performance which results to the choice of this capture technology.

2.
Biol. Res ; 43(4): 385-392, 2010. ilus
Article in English | LILACS | ID: lil-582852

ABSTRACT

Two new cell lines (CCF and CCH) were established from fin and heart tissues of common carp, Cyprinus carpio. The cells were optimally maintained in Leibovitz-15 medium supplemented with 10 percent fetal bovine serum (FBS) and 10 ng/ml of basic fibroblastic growth factor (bFGF). The effects of temperature, concentration of FBS and bFGF on the growth of CCF and CCH cells were examined. The temperature ranged from 24 to 32 °C for good growth of the cells. The growth rate of cells was higher in medium containing 10 percent FBS and the addition of bFGF to the medium significantly increased the growth rate. The CCF cells were found to be epithelial, while the CCH cells were fibroblastic in nature. The cytogenetic analysis of the cell lines revealed a diploid number of 100 chromosomes in C. carpio. The viability of CCF and CCH cell lines were 70 and 72 percent, respectively, after six months of storage in liquid nitrogen (-196 ° C). Molecular characterization of the cell lines using 16S rRNA and Cytochrome Oxidase Subunit I (COI) revealed the origin of the cell lines. These new cell lines will be useful for isolation of fish viruses and other in vitro biotechnological studies.


Subject(s)
Animals , Cattle , Animal Fins/cytology , Carps , Cell Culture Techniques/methods , Cell Line/cytology , Myocardium/cytology , Cell Survival , Cryopreservation , Carps/virology , Karyotyping , /genetics , Temperature , Time Factors
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